Journal: Scientific Reports

Article Title: A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide

doi: 10.1038/s41598-019-42076-2

Figure Lengend Snippet: Antigen/vaccine structures and conjugation procedures used in this work. ( A ) structure of the 16-residue tandem repeat peptide from MUC4 with a TF ag at position Ser5 (5TF ag -TR MUC4 ) and the PEG linker used to conjugate to gold nanoparticles to construct our vaccine platform (shown), as well as to prepare KLH and BSA conjugates ( B ).

Article Snippet: All FFPE materials, including tissue section, TMA, and cell pellet slides, were stained with the same methods, with anti-MUC4 antibody (Rockland 600-401-GY2, Rabbit, 1:1000).

Techniques: Conjugation Assay, Residue, Construct

Journal: Scientific Reports

Article Title: A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide

doi: 10.1038/s41598-019-42076-2

Figure Lengend Snippet: Flow cytometry histograms of pAb binding to MUC4 + and MUC4 − cell lines.

Article Snippet: All FFPE materials, including tissue section, TMA, and cell pellet slides, were stained with the same methods, with anti-MUC4 antibody (Rockland 600-401-GY2, Rabbit, 1:1000).

Techniques: Flow Cytometry, Binding Assay

Journal: Scientific Reports

Article Title: A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide

doi: 10.1038/s41598-019-42076-2

Figure Lengend Snippet: Plot of signal intensity relative to glycan families for pAb (1:1000 dilution) binding the glycan microarray. Only the MUC4 peptides/glycopeptides that printed bound to the serum, while no other TF-containing molecule bound.

Article Snippet: All FFPE materials, including tissue section, TMA, and cell pellet slides, were stained with the same methods, with anti-MUC4 antibody (Rockland 600-401-GY2, Rabbit, 1:1000).

Techniques: Glycoproteomics, Binding Assay, Microarray

Journal: Scientific Reports

Article Title: A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide

doi: 10.1038/s41598-019-42076-2

Figure Lengend Snippet: Staining of various MUC4 + and MUC4 − cell pellets with affinity-purified pAb serum. Some non-specific, acellular staining is seen within MiaPACA2 (MUC4 − ) cells. No staining was observed for control liver and PANC1 (MUC4 − ) cell pellets while HPAFII and HPAC pellets that are known to express large amounts of MUC4 were strongly and diffusely positive.

Article Snippet: All FFPE materials, including tissue section, TMA, and cell pellet slides, were stained with the same methods, with anti-MUC4 antibody (Rockland 600-401-GY2, Rabbit, 1:1000).

Techniques: Staining, Affinity Purification, Control

Journal: Scientific Reports

Article Title: A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide

doi: 10.1038/s41598-019-42076-2

Figure Lengend Snippet: A section of pancreatic adenocarcinoma with vascular invasion is evaluated for MUC4 expression. The primary tumor mass ( A ) is heterogenous for MUC4 expression, with some neoplastic cells demonstrating strong positive staining while others are negative. At higher power, MUC4 staining is intense within some neoplastic cells ( B ) and not expressed in others. Numerous tumor emboli are present within lymphatic vessels in this tissue section; essentially all neoplastic cells invading vascular structures are positive for MUC4, suggesting the antiserum recognizes tumor clones more likely to produce metastasis within the heterogenous neoplastic tissue.

Article Snippet: All FFPE materials, including tissue section, TMA, and cell pellet slides, were stained with the same methods, with anti-MUC4 antibody (Rockland 600-401-GY2, Rabbit, 1:1000).

Techniques: Expressing, Staining, Clone Assay

Journal: Cell Death Discovery

Article Title: NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving

doi: 10.1038/s41420-021-00521-0

Figure Lengend Snippet: A ROS may result in ER-stress. ROS or/and ER-stress incurs JNK activation. The activated JNK leads to pancreatic β cell apoptosis via mitochondria-dependent or independent pathways. B Acute ROS or ER-stress results in the expression of NR4A1, which in turn enhances the expression of MKP7, while MKP7 is able to abolish partial JNK activation by exerting its phosphatase activity, therefore, largely reduces pancreatic β cell apoptosis. The fate of pancreatic β cells depends on the balance between the availability of NR4A1 or MKP7 and the severity of ROS or ER-stress.

Article Snippet: The mouse pancreatic β cell lines (MIN6 and β-TC6) were from ATCC.

Techniques: Activation Assay, Expressing, Activity Assay